TYMS antibody

Catalog No. ABIN107488

Supplier Product No.: 200-601-199-0100

This Sheep Polyclonal antibody specifically detects TYMS in WB, IHC, ELISA and FM. It exhibits reactivity toward Human.

Quick Overview for TYMS antibody (ABIN107488)

Target: TYMS (Thymidylate Synthetase (TYMS))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This TYMS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Product Details anti-TYMS Antibody

Supplier Product No.: 200-601-199-0100

Supplier: Rockland

Purpose: Thymidylate Synthase Antibody

Cross-Reactivity (Details): Because inhibition of Thymidylate Synthase prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors.

Characteristics: Synonyms: sheep anti-Thymidylate Synthase Antibody, d TMP synthase antibody, EC 2.1.1.45 antibody, HsT422 antibody, MGC88736 antibody

Purification: Anti-THYMIDYLATE SYNTHASE IgG fraction is directed against human Thymidylate Synthase and is useful in determining its presence in various assays.

Immunogen:

Immunogen: Anti-THYMIDYLATE SYNTHASE was purified from rabbit serum after repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: THYMIDYLATE SYNTHASE antibody has been tested by western blot. This antibody is suitable for use in ELISA, immunoprecipitation, immunofluorescence microscopy, and immunohistochemistry.  The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR.  This event occurs in most human breast, colorectal, gastric, head and neck carcinomas.  The antibody recognizes the 36,000 MW hTS.  Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: TYMS

Background: Background: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript rTSalpha vary inversely when cell-growth progresses from late-log to plateau phase. Diseases associated with Thymidylate synthase include Rectal Neoplasm and Dihydropyrimidine Dehydrogenase Deficiency. Anti-Thymidylate synthase is useful for researchers interested in Circadian Rhythm, Metabolism and cell cycle research.

Gene ID: 7298

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases